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Addgene

pHSG+PTDH
(Plasmid #66184)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66184 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHSG398
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 2206
  • Total vector size (bp) 3225
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phosphite Dehydrogenase
  • Alt name
    ptxD
  • Alt name
    phosphonate dehydrogenase
  • Alt name
    PTDH
  • Species
    Synthetic; Pseudomonas
  • Insert Size (bp)
    1019
  • Mutation
    E175A, A176R
  • GenBank ID
    WP_003118429

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 (-40)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Watanabe et al Chemcatchem. 2013 Dec;5(12):3835-40. doi: 10.1002/cctc.201300445.
The phosphite Dehydrogenase gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHSG+PTDH was a gift from Teruyuki Nagamune (Addgene plasmid # 66184 ; http://n2t.net/addgene:66184 ; RRID:Addgene_66184)

  • For your References section:

    Phosphite-driven Self-sufficient Cytochrome P450. Watanabe H, Hirakawa H, and Nagamune T.. ChemCatChem 2013 Dec;5(12):3835-3840 10.1002/cctc.201300445
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