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Addgene

pPTDH
(Plasmid #66183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6471
  • Modifications to backbone
    A SapI recognition site (GAAGAGC) was modified to GTTGAGC.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Phosphite Dehydrogenase
  • Alt name
    ptxD
  • Alt name
    phosphonate dehydrogenase
  • Alt name
    PTDH
  • Species
    Synthetic; Pseudomonas
  • Insert Size (bp)
    1043
  • Mutation
    E175A, A176R
  • GenBank ID
    WP_003118429
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His6 tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Watanabe et al Chemcatchem. 2013 Dec;5(12):3835-40. doi: 10.1002/cctc.201300445.
The phosphite Dehydrogenase gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPTDH was a gift from Teruyuki Nagamune (Addgene plasmid # 66183 ; http://n2t.net/addgene:66183 ; RRID:Addgene_66183)

  • For your References section:

    Phosphite-driven Self-sufficient Cytochrome P450. Watanabe H, Hirakawa H, and Nagamune T.. ChemCatChem 2013 Dec;5(12):3835-3840 10.1002/cctc.201300445
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