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Addgene

pBabe-SIRT3
(Plasmid #66167)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66167 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe puro
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIRT3
  • Alt name
    Sirtuin 3
  • Species
    M. musculus (mouse)
  • Mutation
    L235P
  • Entrez Gene
    Sirt3 (a.k.a. 2310003L23Rik, Sir2l3)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-SIRT3 was a gift from Ronald Kahn (Addgene plasmid # 66167 ; http://n2t.net/addgene:66167 ; RRID:Addgene_66167)

  • For your References section:

    Sirt3 regulates metabolic flexibility of skeletal muscle through reversible enzymatic deacetylation. Jing E, O'Neill BT, Rardin MJ, Kleinridders A, Ilkeyeva OR, Ussar S, Bain JR, Lee KY, Verdin EM, Newgard CB, Gibson BW, Kahn CR. Diabetes. 2013 Oct;62(10):3404-17. doi: 10.2337/db12-1650. Epub 2013 Jul 8. 10.2337/db12-1650 PubMed 23835326
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