FoxO1 KQ
(Plasmid #66165)

• Purpose: Expresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by glutamine residues.
• Depositing Lab: Ronald Kahn
• Publication: Jing et al Cell Metab. 2007 Aug . 6(2):105-14.

Backbone

• Vector backbone: pBabe bleo
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FoxO1 KQ
• Alt name: FKHR
• Species: M. musculus (mouse)
• Mutation: K242Q, K245Q and K262Q
• Entrez Gene: Foxo1 (a.k.a. Afxh, FKHR, Fkhr1, Foxo1a)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBABE5
• 3′ sequencing primer: pBABE3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry