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Addgene

pCVD630
(Plasmid #66163)

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Item Catalog # Description Quantity Price (USD)
Plasmid 66163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCVD315
  • Backbone manufacturer
    Kaper lab, Addgene plasmid 63737
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ace
  • Alt name
    accessory cholera enterotoxin
  • Insert Size (bp)
    2900
  • Promoter Unknown

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned ace gene from El tor Vibrio cholerae E7946 on a 2.9 Kb EcoRV fragment cloned into pCVD315. Also contains 391 bp of zot.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD630 was a gift from James Kaper (Addgene plasmid # 66163 ; http://n2t.net/addgene:66163 ; RRID:Addgene_66163)

  • For your References section:

    Accessory cholera enterotoxin (Ace), the third toxin of a Vibrio cholerae virulence cassette. Trucksis M, Galen JE, Michalski J, Fasano A, Kaper JB. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5267-71. PubMed 8389476
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