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Addgene

pCVD621
(Plasmid #66162)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCVD315
  • Backbone manufacturer
    Kaper lab, Addgene plasmid 63737
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ctxB
  • Insert Size (bp)
    1400
  • Promoter Unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pBRforEco
  • 3′ sequencing primer MoCloF
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ctxB with KpnI linkers cloned into pCVD315 KpnI site. 1.4 kb ctxB probe.plasmid GENOTYPE :pCVD315::ctxB

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD621 was a gift from James Kaper (Addgene plasmid # 66162 ; http://n2t.net/addgene:66162 ; RRID:Addgene_66162)

  • For your References section:

    CVD110, an attenuated Vibrio cholerae O1 El Tor live oral vaccine strain. Michalski J, Galen JE, Fasano A, Kaper JB. Infect Immun. 1993 Oct;61(10):4462-8. PubMed 8406837
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