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Addgene

pCVD619
(Plasmid #66161)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 66161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nag-ST
  • Alt name
    heat-stable enterotoxin
  • Insert Size (bp)
    11000
  • Promoter Unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer pBRforEco
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBR322::NAG-ST (11kb Bg1II insert) Cloned heat-stable enterotoxin from parent strain Vibrio cholera NRT36S.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD619 was a gift from James Kaper (Addgene plasmid # 66161 ; http://n2t.net/addgene:66161 ; RRID:Addgene_66161)
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