pCVD453
(Plasmid
#66154)
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Purposecloning of E. coli E2348/69 left end of the LEE region, contains rorf1 and espG.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSPORT1
- Backbone size w/o insert (bp) 4109
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLEE probe A rorf1, espG
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Insert Size (bp)3100
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E. coli E2348, 3.1 Kb Bg1II-MluI fragment from the left end of the LEE region cloned into the BamHI-MluI site of vector pSPORT I. Contains rorf1 and espG. parent strain: E. coli E2348/69
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD453 was a gift from James Kaper (Addgene plasmid # 66154 ; http://n2t.net/addgene:66154 ; RRID:Addgene_66154) -
For your References section:
A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. McDaniel TK, Jarvis KG, Donnenberg MS, Kaper JB. Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1664-8. PubMed 7878036