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Addgene

pCVD450
(Plasmid #66153)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC184
  • Backbone size w/o insert (bp) 4300
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    perA
  • Promoter none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CAT_int
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E coli E2348/69 PerA plasmid regulator. perA subclone from pMAR2. ~ 5 kb EcoRI fragment of pMAR2 (GenBank accession number FM180569.1) containing perA, perB and perC cloned into the EcoRI site of pACYC184.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD450 was a gift from James Kaper (Addgene plasmid # 66153 ; http://n2t.net/addgene:66153 ; RRID:Addgene_66153)

  • For your References section:

    Studies on the regulation of virulence gene expression of enteropathogenic Escherichia coli. Gomez OG. University of Maryland, Baltimore. Dissertation (1993). http://hdl.handle.net/10713/1623
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