pCVD443
(Plasmid
#66150)
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Purposecloning of EPEC E.coli eae gene
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeae
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Alt nameLEE probe C
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Alt nameE. coli attaching and effacing
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Insert Size (bp)1000
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E. coli E2348/69 cloned intimin. HindIII restriction site added to the ends of eae gene (1 kb) by PCR and cloned into pUC19.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD443 was a gift from James Kaper (Addgene plasmid # 66150 ; http://n2t.net/addgene:66150 ; RRID:Addgene_66150) -
For your References section:
A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. McDaniel TK, Jarvis KG, Donnenberg MS, Kaper JB. Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1664-8. PubMed 7878036