Skip to main content
Addgene

pCVD404
(Plasmid #66149)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66149 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBR325
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5996
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STp
  • Alt name
    est (porcine)
  • Alt name
    Heat stable enterotoxin gene (porcine)
  • Insert Size (bp)
    157
  • Promoter none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer pBR322ori-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains the E. coli STp gene probe (157 bp) cloned on PstI linkers into pBR325 Heat stable enterotoxin gene (porcine)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD404 was a gift from James Kaper (Addgene plasmid # 66149 ; http://n2t.net/addgene:66149 ; RRID:Addgene_66149)

  • For your References section:

    Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test. Nishibuchi M, Arita M, Honda T, Miwatani T. J Clin Microbiol. 1988 Apr;26(4):784-6. PubMed 3284902
Related items: