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pCVD402
(Plasmid #66148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR325
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5996
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STh
  • Alt name
    est (human)
  • Alt name
    Heat stable enterotoxin gene (human)
  • Insert Size (bp)
    216
  • Promoter none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CAT_int
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

est gene probe (216 bp) cloned on EcoRI linkers into pBR325. STh = Heat stable enterotoxin gene (human) from ETEC E. coli 153837-2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD402 was a gift from James Kaper (Addgene plasmid # 66148 ; http://n2t.net/addgene:66148 ; RRID:Addgene_66148)

  • For your References section:

    Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test. Nishibuchi M, Arita M, Honda T, Miwatani T. J Clin Microbiol. 1988 Apr;26(4):784-6. PubMed 3284902
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