pCVD402
(Plasmid
#66148)
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Purposeto make a probe to detect Heat stable enterotoxin gene (human)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR325
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5996
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSTh
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Alt nameest (human)
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Alt nameHeat stable enterotoxin gene (human)
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Insert Size (bp)216
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CAT_int (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
est gene probe (216 bp) cloned on EcoRI linkers into pBR325. STh = Heat stable enterotoxin gene (human) from ETEC E. coli 153837-2
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD402 was a gift from James Kaper (Addgene plasmid # 66148 ; http://n2t.net/addgene:66148 ; RRID:Addgene_66148) -
For your References section:
Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test. Nishibuchi M, Arita M, Honda T, Miwatani T. J Clin Microbiol. 1988 Apr;26(4):784-6. PubMed 3284902