pBabe-SIRT2
(Plasmid
#66144)
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PurposeExpresses SIRT2 via retroviral method
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBabe puro
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSIRT2
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Alt nameSirtuin 2
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SpeciesM. musculus (mouse)
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MutationE28G
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Entrez GeneSirt2 (a.k.a. 5730427M03Rik, SIR2L2, Sir2l)
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBABE5
- 3′ sequencing primer pBABE3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-SIRT2 was a gift from Ronald Kahn (Addgene plasmid # 66144 ; http://n2t.net/addgene:66144 ; RRID:Addgene_66144) -
For your References section:
SIRT2 regulates adipocyte differentiation through FoxO1 acetylation/deacetylation. Jing E, Gesta S, Kahn CR. Cell Metab. 2007 Aug . 6(2):105-14. 10.1016/j.cmet.2007.07.003 PubMed 17681146