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Addgene

pET11+PCNA3-CYP119(T214V)
(Plasmid #66137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5639
  • Total vector size (bp) 7567
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Supplement with 1% glucose is recommended to suppress leaky expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CYP119
  • Insert Size (bp)
    1928
  • Mutation
    T214V
  • Entrez Gene
    cyp119 (a.k.a. Saci_2081)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • Sulfolobus solfataricus PCNA3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11+PCNA3-CYP119(T214V) was a gift from Teruyuki Nagamune (Addgene plasmid # 66137 ; http://n2t.net/addgene:66137 ; RRID:Addgene_66137)

  • For your References section:

    Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins. Suzuki R, Hirakawa H, Nagamune T. Biotechnol J. 2014 Dec;9(12):1573-81. doi: 10.1002/biot.201400007. Epub 2014 Jul 21. 10.1002/biot.201400007 PubMed 24924478
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