pET11a+CYP119
(Plasmid #66131)

• Purpose: Expresses the wild type of CYP119
• Depositing Lab: Teruyuki Nagamune
• Publication: Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. doi: 10.1002/biot.201400007. Epub 2014 Jul 21.

Backbone

• Vector backbone: pET-11a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5639
• Total vector size (bp): 6748
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CYP119
• Insert Size (bp): 1109
• Entrez Gene: cyp119 (a.k.a. Saci_2081)
• Promoter: T7 promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).