pGL4.10-VEGFprom(-1000 to -1)
(Plasmid #66128)

• Purpose: Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)
• Depositing Lab: David Mu
• Publication: Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.1038/srep19857.

Backbone

• Vector backbone: pGL4.10
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4242
• Total vector size (bp): 5242
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vascular Endothelial Growth Factor -A promoter region
• Alt name: VEGF
• Alt name: VEGFA
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Entrez Gene: VEGFA (a.k.a. L-VEGF, MVCD1, VEGF, VPF)
• Promoter: VEGF -1000 to -1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: TTAAGGTACCCCAGTCCCAAATATGTAGCTGTT
• 3′ sequencing primer: TTAAAAGCTTCCACAGTGATTTGGGGAAGTA

Resource Information

• Supplemental Documents:
  • Plasmid Design VEGFprom(1000-1)
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry