pHSG-PCNA3
(Plasmid #66127)

• Purpose: S. solfataricus PCNA3
• Depositing Lab: Teruyuki Nagamune
• Publication: Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20. doi: 10.1002/cbic.201000226.

Backbone

• Vector backbone: pHSG398
• Backbone manufacturer: TaKaRa
• Backbone size w/o insert (bp): 2205
• Total vector size (bp): 3003
• Vector type: Cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DNA polymerase sliding clamp 3
• Alt name: PCNA3
• Species: Sulfolobus solfataricus P2
• Insert Size (bp): 798
• GenBank ID: NP_341944
• Entrez Gene: pcn (a.k.a. SSO_RS02035, SSO0405)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13 (-40)
• 3′ sequencing primer: M13 Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We cloned the PCNA3 gene from the cell suspension of S. solfataricus P2 strain (ATCC35092).