Skip to main content
Addgene

pHSG-PCNA1
(Plasmid #66125)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHSG398
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 2206
  • Total vector size (bp) 2970
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNA polymerase sliding clamp 1
  • Alt name
    PCNA1
  • Species
    Sulfolobus solfataricus P2
  • Insert Size (bp)
    764
  • GenBank ID
    NP_341936
  • Entrez Gene
    SSO_RS01995 (a.k.a. SSO_RS01995, SSO0397)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 (-40)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We cloned the PCNA1 gene from the cell suspension of S. solfataricus P2 strain (ATCC35092).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHSG-PCNA1 was a gift from Teruyuki Nagamune (Addgene plasmid # 66125 ; http://n2t.net/addgene:66125 ; RRID:Addgene_66125)

  • For your References section:

    Molecular assembly of P450 with ferredoxin and ferredoxin reductase by fusion to PCNA. Hirakawa H, Nagamune T. Chembiochem. 2010 Jul 26;11(11):1517-20. doi: 10.1002/cbic.201000226. 10.1002/cbic.201000226 PubMed 20607777
Related items:
Commonly requested with: