YFP(1-158)-STOP
(Plasmid #66107)

• Purpose: This vector attaches YFP(1-158) to the C-terminus of a protein
• Depositing Lab: Catherine Berlot
• Publication: Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-35. doi: 10.1074/jbc.M112.437368. Epub 2013 Mar 12.

Backbone

• Vector backbone: pcDNAI/mp
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4800
• Total vector size (bp): 5242
• Modifications to backbone: A BglII site was added to the polylinker 3' to the BamHI site.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFP(1-158)STOP
• Species: Aequorea victoria
• Insert Size (bp): 483
• Mutation: Gln69 was changed to Met.
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

YFP(1-158)Stop was amplified by PCR from EYFP (Clontech).