dg264_pENTR_ChronosCel
(Plasmid #66102)

• Purpose: Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2
• Depositing Lab: Dominique Glauser
• Publication: Schild et al Genetics. 2015 May 28. pii: genetics.115.177956.

Backbone

• Vector backbone: pUC57-Kan
• Total vector size (bp): 3927
• Modifications to backbone: The insert in this vector is flanked by attL1 and attL2 sites and is suitable for LR recombination reactions (Gateway system).
• Vector type: Gateway

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chronos
• Alt name: ChronosCel
• Species: C. elegans (nematode)
• Insert Size (bp): 1128

Resource Information

• Supplemental Documents:
  • dg264_Chronos Entry Clone in pUC57-Kan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct was produced through de novo gene synthesis.

Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.