G protein alpha-q-CFP
(Plasmid #66081)

• Purpose: This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
• Depositing Lab: Catherine Berlot
• Publication: Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35. Epub 2000 Nov 13.

Backbone

• Vector backbone: pcDNAI/Amp
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4800
• Total vector size (bp): 7119
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein alpha-q-EE-CFP
• Alt name: Gnaq
• Species: M. musculus (mouse)
• Insert Size (bp): 2319
• Mutation: BamHI and SacI sites in alpha-q were removed with silent mutations. EYFP contains substitution of His for Asn164.
• Entrez Gene: Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
• Promoter: CMV
• Tags / Fusion Proteins:
  • CFP was inserted internally into the alpha-q sequence between residues 124 and 125 with a SGGGGS linker on each end of the YFP.
  • The EE epitope was introduced into the alpha-q sequence by mutating residues 171-176 (SYLPTQ) to EYMPTE.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pECFP was purchased from Clontech.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry