pETDuet-1∆HisTag_Nef_MCS2
(Plasmid
#66079)
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PurposeSingle vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli system
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66079 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepETDuet-1
- Backbone size w/o insert (bp) 5420
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNef
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Alt nameStamm SF2
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GenBank IDAAB59883.1
- Promoter T7-lacO
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Tags
/ Fusion Proteins
- HRV 3C Site (C terminal on insert)
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site MunI (unknown if destroyed)
- 5′ sequencing primer pBRrevBam
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETDuet-1∆HisTag_Nef_MCS2 was a gift from Dieter Willbold (Addgene plasmid # 66079 ; http://n2t.net/addgene:66079 ; RRID:Addgene_66079) -
For your References section:
Single vector system for efficient N-myristoylation of recombinant proteins in E. coli. Gluck JM, Hoffmann S, Koenig BW, Willbold D. PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081. 10.1371/journal.pone.0010081 PubMed 20404920
Map uploaded by the depositor.
