pETDuet-1∆HisTag_hNMT_Nef
(Plasmid #66078)

• Purpose: Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system
• Depositing Lab: Dieter Willbold
• Publication: Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081.

Backbone

• Vector backbone: pETDuet-1
• Backbone size w/o insert (bp): 5420
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NMT
• Species: H. sapiens (human)
• Mutation: Deletion of first 80 amino acids
• Entrez Gene: NMT1 (a.k.a. HsNMT1, NMT)
• Promoter: T7-lacO

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: pBRrevBam
• 3′ sequencing primer: T7 Terminal

Gene/Insert 2

• Gene/Insert name: Nef
• Alt name: Stamm SF2
• GenBank ID: AAB59883.1
• Promoter: T7-lacO

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: MunI (unknown if destroyed)
• 5′ sequencing primer: AmpR
• 3′ sequencing primer: pBR322ori-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry