Burden Monitor phi80 version
(Plasmid #66074)

• Purpose: The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell
• Depositing Lab: Tom Ellis
• Publication: Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.

Backbone

• Vector backbone: pAH153
• Backbone size w/o insert (bp): 2323
• Total vector size (bp): 3162

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Growth instructions: PIR+ strain is required for R6K conditional origin of replication
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: Burden Monitor: constitutive BBa_J23100 promoter-strong RBS- superfolder GFP without tag- BBa_1002 terminator
• Insert Size (bp): 828

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry