Burden Monitor lambda version
(Plasmid
#66073)
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PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAH63
- Backbone size w/o insert (bp) 2455
- Total vector size (bp) 3303
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Growth instructionsPIR+ strain is required for R6K conditional origin of replication
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP
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Alt nameBurden Monitor: constitutive BBa_J23100 promoter-strong RBS- superfolder GFP without tag- BBa_1002 terminator
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Insert Size (bp)829
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Burden Monitor lambda version was a gift from Tom Ellis (Addgene plasmid # 66073 ; http://n2t.net/addgene:66073 ; RRID:Addgene_66073) -
For your References section:
Quantifying cellular capacity identifies gene expression designs with reduced burden. Ceroni F, Algar R, Stan GB, Ellis T. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6. 10.1038/nmeth.3339 PubMed 25849635
Map uploaded by the depositor.
