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Addgene

Burden Monitor lambda version
(Plasmid #66073)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAH63
  • Backbone size w/o insert (bp) 2455
  • Total vector size (bp) 3303

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    PIR+ strain is required for R6K conditional origin of replication
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    Burden Monitor: constitutive BBa_J23100 promoter-strong RBS- superfolder GFP without tag- BBa_1002 terminator
  • Insert Size (bp)
    829

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Burden Monitor lambda version was a gift from Tom Ellis (Addgene plasmid # 66073 ; http://n2t.net/addgene:66073 ; RRID:Addgene_66073)

  • For your References section:

    Quantifying cellular capacity identifies gene expression designs with reduced burden. Ceroni F, Algar R, Stan GB, Ellis T. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6. 10.1038/nmeth.3339 PubMed 25849635
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