234_pSKB3-Venus-LUMP
(Plasmid #65975)

• Purpose: Bacterial expression of Venus-LUMP FRET pair
• Depositing Lab: Gerard Marriott
• Publication: Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.

Backbone

• Vector backbone: pSKB3
• Backbone size w/o insert (bp): 5245
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LUMP
• Species: Photobacterium leignathi
• Insert Size (bp): 1353
• Promoter: T7
• Tag / Fusion Protein:
  • Venus (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pSKB3-Venus-LUMP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The fluorescent protein sequence matches Citrine, which has very similar spectra to Venus. This should have no effect on plasmid function, but the plasmid name is maintained to correspond to the associated publication.