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213-petDuet1-GBD-LUMP--cdc42
(Plasmid #65973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    petDuet1
  • Backbone size w/o insert (bp) 5321
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LUMP
  • Species
    Photobacterium leignathi
  • Insert Size (bp)
    702
  • Promoter T7
  • Tag / Fusion Protein
    • GBD (GTPase binding domain from ACK1; residues 448–489) (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    cell division cycle 42
  • Species
    H. sapiens (human)
  • Mutation
    constitutively active Cdc42 (Q61L)
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    213-petDuet1-GBD-LUMP--cdc42 was a gift from Gerard Marriott (Addgene plasmid # 65973 ; http://n2t.net/addgene:65973 ; RRID:Addgene_65973)

  • For your References section:

    Genetically encoded sensors of protein hydrodynamics and molecular proximity. Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G. Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021. 10.1073/pnas.1424021112 PubMed 25931526
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