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Addgene

psicheck_NDUFA6_3UTR_mut
(Plasmid #65971)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-2
  • Backbone manufacturer
    Promega, modified by the Tuschl lab
  • Backbone size w/o insert (bp) 6249
  • Modifications to backbone
    DEST-LR: includes LR clonase sites to introduce insert
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NDUFA6
  • Species
    H. sapiens (human)
  • Mutation
    mutated tandem CAC motif to CCC motif
  • GenBank ID
  • Entrez Gene
    NDUFA6 (a.k.a. B14, CI-B14, LYRM6, NADHB14)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutagenesis of Addgene plasmid 65886 using following primers

GCA CGT TGA TAC TAT TTT AGA GCC CAA ATA AAC TCC CTA TAC AAT GG

CCA TTG TAT AGG GAG TTT ATT TGG GCT CTA AAA TAG TAT CAA CGT GC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psicheck_NDUFA6_3UTR_mut was a gift from Thomas Tuschl (Addgene plasmid # 65971 ; http://n2t.net/addgene:65971 ; RRID:Addgene_65971)
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