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Addgene

p(bhsp68-Cas9)
(Plasmid #65959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLfa[Tc-bhsp68::nlsEGFP]fa
  • Backbone size w/o insert (bp) 4168
  • Total vector size (bp) 8323
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4155
  • Promoter bhsp
  • Tag / Fusion Protein
    • nuclear localization sequence (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer TAACCGGTCGCCACCATGGATAAGAAATAC
  • 3′ sequencing primer AAGCGGCCGCTCATCCTGCAGCTCCAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cas9 was cloned from Addgene MLM3613
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An online tool to help oligo design for gRNAs is at http://www.averof-lab.org/tools/Tribolium_CRISPR.php

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p(bhsp68-Cas9) was a gift from Michalis Averof (Addgene plasmid # 65959 ; http://n2t.net/addgene:65959 ; RRID:Addgene_65959)

  • For your References section:

    Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum. Gilles AF, Schinko JB, Averof M. Development. 2015 Jul 9. pii: dev.125054. 10.1242/dev.125054 PubMed 26160901
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