GIV-F1685A FLAG
(Plasmid
#65948)
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PurposeExpression GIV/Girdin F1685A mutation in Mammalian cell
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65948 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3XFLAG-CMV-14
- Total vector size (bp) 6300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGIV/Girdin
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Alt nameGIV
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SpeciesH. sapiens (human)
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Insert Size (bp)5610
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MutationPhenylalanine 1685 to Alanine
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GenBank IDBAE44387.1, AB201172.1
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Entrez GeneCCDC88A (a.k.a. APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFlag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not I (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMVf (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GIV-F1685A FLAG was a gift from Pradipta Ghosh (Addgene plasmid # 65948 ; http://n2t.net/addgene:65948 ; RRID:Addgene_65948) -
For your References section:
Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin. Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, Kufareva I, Abagyan R, Ghosh P. Mol Biol Cell. 2014 Nov 5;25(22):3654-71. doi: 10.1091/mbc.E14-05-0978. Epub 2014 Sep 3. 10.1091/mbc.E14-05-0978 PubMed 25187647