pTac-A-luci
(Plasmid
#65933)
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PurposeTheophylline Riboswitch A with luciferase gene
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameluciferase
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SpeciesSynthetic
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Insert Size (bp)1800
- Promoter Tac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site PstI (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTac-A-luci was a gift from Justin Gallivan (Addgene plasmid # 65933 ; http://n2t.net/addgene:65933 ; RRID:Addgene_65933) -
For your References section:
A family of synthetic riboswitches adopts a kinetic trapping mechanism. Mishler DM, Gallivan JP. Nucleic Acids Res. 2014 Jun;42(10):6753-61. doi: 10.1093/nar/gku262. Epub 2014 Apr 29. 10.1093/nar/gku262 PubMed 24782524