Skip to main content
Addgene

pBAV-A
(Plasmid #65927)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBAV1K
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Plasmid also functions in bacterial species A. baylyi
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lacZ
  • Alt name
    beta-galactosidase
  • Species
    Synthetic
  • Insert Size (bp)
    3200
  • Promoter T5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site SpeI (unknown if destroyed)
  • 5′ sequencing primer cgcggccgcttctagaggaaatc
  • 3′ sequencing primer CGGCCTCCGCAGGAAGCCGTTTTTTTCG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Colleen Reynoso

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAV-A was a gift from Justin Gallivan (Addgene plasmid # 65927 ; http://n2t.net/addgene:65927 ; RRID:Addgene_65927)

  • For your References section:

    Synthetic riboswitches that induce gene expression in diverse bacterial species. Topp S, Reynoso CM, Seeliger JC, Goldlust IS, Desai SK, Murat D, Shen A, Puri AW, Komeili A, Bertozzi CR, Scott JR, Gallivan JP. Appl Environ Microbiol. 2010 Dec;76(23):7881-4. doi: 10.1128/AEM.01537-10. Epub 2010 Oct 8. 10.1128/AEM.01537-10 PubMed 20935124
Related items:
Commonly requested with: