psicheck_200_300_CA_repeat
(Plasmid
#65880)
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PurposeLuciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepsiCHECK-2
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Backbone manufacturerPromega, modified by the Tuschl lab
- Backbone size w/o insert (bp) 6249
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Modifications to backboneDEST-LR: includes LR clonase sites to introduce insert
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCA repeats 200-300 nucleotides in length
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Insert Size (bp)250
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 EEV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LR recombinase reaction from Addgene plasmid 65869
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psicheck_200_300_CA_repeat was a gift from Thomas Tuschl (Addgene plasmid # 65880 ; http://n2t.net/addgene:65880 ; RRID:Addgene_65880)