pDONR221_50_100_CA_repeat
(Plasmid
#65867)
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PurposeCarries CA repeats of 50-100 nucleotides in length, an RBPMS target
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies, modified by the Tuschl lab
- Backbone size w/o insert (bp) 2550
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCA repeats 50-100 nucleotides in lngth
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Insert Size (bp)75
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_50_100_CA_repeat was a gift from Thomas Tuschl (Addgene plasmid # 65867 ; http://n2t.net/addgene:65867 ; RRID:Addgene_65867)