Skip to main content
Addgene

pDONR221_ETF1_3UTR
(Plasmid #65865)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Life Technologies, modified by the Tuschl lab
  • Backbone size w/o insert (bp) 2550

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ETF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2210
  • Entrez Gene
    ETF1 (a.k.a. D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1)
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Primers used to amplify 3' UTR from human cDNA (HCC1964 cDNA):
ETF1 forward: AAA AAG CAG GCT TCG TAG TCG ACA TGG GTC CGG
ETF1 reverse: AGA AAG CTG GGT GTA TTC TTA AAT ATG GTT TAA TAC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221_ETF1_3UTR was a gift from Thomas Tuschl (Addgene plasmid # 65865 ; http://n2t.net/addgene:65865 ; RRID:Addgene_65865)
Related items: