pDONR221_CDK2AP1_3UTR
(Plasmid
#65862)
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PurposeCarries 3' UTR of CDK2AP1, a miR-21 target
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies, modified by the Tuschl lab
- Backbone size w/o insert (bp) 2550
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCDK2AP1
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SpeciesH. sapiens (human)
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GenBank IDNM_004642
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Entrez GeneCDK2AP1 (a.k.a. DOC1, DORC1, ST19, doc-1, p12DOC-1)
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_CDK2AP1_3UTR was a gift from Thomas Tuschl (Addgene plasmid # 65862 ; http://n2t.net/addgene:65862 ; RRID:Addgene_65862)