MS2-Snapf
(Plasmid
#65860)
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PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 65860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3400
- Total vector size (bp) 8000
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Modifications to backboneRemoved GFP and inserted MS2 and SNAPf
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameMS2
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Speciesbacteriophage MS2
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- NLS (C terminal on insert)
Gene/Insert 2
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Gene/Insert nameFast SNAP
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Alt nameSNAPf
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MS2-Snapf was a gift from Aaron Hoskins (Addgene plasmid # 65860 ; http://n2t.net/addgene:65860 ; RRID:Addgene_65860) -
For your References section:
Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores. Carrocci TJ, Hoskins AA. Analyst. 2014 Jan 7;139(1):44-7. doi: 10.1039/c3an01550e. Epub 2013 Nov 1. 10.1039/c3an01550e PubMed 24187681
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/65/65860/65860-map_I_Geq_QxAEev.pdf.940x940_q85_autocrop.png)