Skip to main content
Addgene

MS2-ecDHFR
(Plasmid #65859)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3400
  • Total vector size (bp) 8000
  • Modifications to backbone
    Removed GFP and inserted MS2 and DHFR
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    MS2
  • Species
    bacteriophage MS2
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • NLS (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    E. coli Dihydrofolate Reductase
  • Alt name
    eDHFR
  • Entrez Gene
    folA (a.k.a. b0048, ECK0049, tmr, tmrA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MS2-ecDHFR was a gift from Aaron Hoskins (Addgene plasmid # 65859 ; http://n2t.net/addgene:65859 ; RRID:Addgene_65859)

  • For your References section:

    Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores. Carrocci TJ, Hoskins AA. Analyst. 2014 Jan 7;139(1):44-7. doi: 10.1039/c3an01550e. Epub 2013 Nov 1. 10.1039/c3an01550e PubMed 24187681
Related items:
Commonly requested with: