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Addgene

pMB1610_pRR-Puro
(Plasmid #65853)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65853 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    phRL-SV40
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3705
  • Total vector size (bp) 3583
  • Modifications to backbone
    Renilla Luciferase coding sequence replaced with split puromycin N-acetyltransferase
  • Vector type
    Mammalian Expression, CRISPR, TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    split puromycin N-acetyltransferase coding sequence
  • Alt name
    split PuroR
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site AatII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB1610_pRR-Puro was a gift from Marc Bühler (Addgene plasmid # 65853 ; http://n2t.net/addgene:65853 ; RRID:Addgene_65853)

  • For your References section:

    Single-Step Generation of Conditional Knockout Mouse Embryonic Stem Cells. Flemr M, Buhler M. Cell Rep. 2015 Jul 28;12(4):709-16. doi: 10.1016/j.celrep.2015.06.051. Epub 2015 Jul 16. 10.1016/j.celrep.2015.06.051 PubMed 26190102
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