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Addgene

pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
(Plasmid #65846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA DEST 47
  • Total vector size (bp) 6252
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transmembrane (23 AA) of UQCC3
  • Alt name
    C11orf83
  • Species
    H. sapiens (human)
  • Mutation
    R5A-K6A
  • GenBank ID
    NM_001085372.2
  • Entrez Gene
    UQCC3 (a.k.a. C11orf83, CCDS41658.1, MC3DN9, UNQ655)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer CACCATGGATTCCTTGCGGAAAATGCTGATCTC
  • 3′ sequencing primer CGCGTAGCCCACGCCAGCCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP was a gift from Amos Bairoch (Addgene plasmid # 65846 ; http://n2t.net/addgene:65846 ; RRID:Addgene_65846)

  • For your References section:

    C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization. Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L. Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. Epub 2015 Jan 20. 10.1128/MCB.01047-14 PubMed 25605331
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