XW83
(Plasmid #65841)

• Purpose: MosSCI-Pmig-13::QF::SL2::mCherry
• Depositing Lab: Kang Shen
• Publication: Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.

Backbone

• Vector backbone: CM290 (a derivative of pCFJ178)
• Backbone manufacturer: C. Maeder
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OR74A quinic acid utilization activator (qa-1F)
• Alt name: QF
• Species: Neurospora crassa
• Insert Size (bp): 2451
• Mutation: The endogenous SphI, KpnI and SacI sites in QF are removed or the convenience of cloning, most sites are compatible with pPD95.77
• GenBank ID: XM_954525.2
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Pmig-13::QF::SL2::mCherry fragment from XW43 was subcloned into SphI and PacI sites of CM290 (a derivative of pCFJ178, with extra cloning sites, a kind gift from C. Maeder), which is the chromosome IV cxTi10882 site MosSCI targeting vector