Skip to main content
Addgene

XW82
(Plasmid #65840)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    CM224 (a derivative of pCFJ151)
  • Backbone manufacturer
    C. Maeder
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5XQUAS-delta-pes-10::GFP
  • Alt name
    QUAS::GFP
  • Species
    Neurospora crassa
  • Insert Size (bp)
    1289
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

QUAS-Δpes-10-GFP fragment from XW12 was subcloned into SphI and PacI sites of CM224 (a derivative of pCFJ151, with extra cloning sites, a kind gift from C. Maeder), which is the chromosome II ttTi5605 site MosSCI targeting vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XW82 was a gift from Kang Shen (Addgene plasmid # 65840 ; http://n2t.net/addgene:65840 ; RRID:Addgene_65840)

  • For your References section:

    Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans. Wei X, Potter CJ, Luo L, Shen K. Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929. 10.1038/nmeth.1929 PubMed 22406855
Related items:
Commonly requested with: