XW17
(Plasmid #65835)

• Purpose: Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)
• Depositing Lab: Kang Shen
• Publication: Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.

Backbone

• Vector backbone: pSM
• Backbone manufacturer: C.I. Bargmann and S. MaCarroll
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 9862
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OR74A quinic acid utilization activator (qa-1F)
• Alt name: QF
• Species: Neurospora crassa
• Insert Size (bp): 2451
• Mutation: The endogenous SphI, KpnI and SacI sites in QF are removed or the convenience of cloning, most sites are compatible with pPD95.77
• GenBank ID: XM_954525.2
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed or the convenience of cloning, most sites are compatible with pPD95.77)