XW12
(Plasmid #65834)

• Purpose: Pquas-Δpes10-GFP-3'UTR-unc-54
• Depositing Lab: Kang Shen
• Publication: Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.

Backbone

• Vector backbone: pSM
• Backbone manufacturer: C.I. Bargmann and S. MaCarroll
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 4688
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5XQUAS-delta-pes-10
• Alt name: QUAS
• Species: Neurospora crassa
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5xQUAS-hsp70 was amplified from pQUAS-CD8-GFP3 with primers 5’- ACTTACTTGCATGCGGATCCGGGTAATCGCTTA and 3’- AGTGGCGCGCCCAATTCCCTATTCAGAGTTC, and the fragment was inserted into SphI and AscI sites of pSM-GFP. Δpes-10 minimal promoter was amplified from pPD97.78 (A. Fire) with primers 5’- GCAAGTGATATCCCTGCAGGATCGATTTT
TTGCA and 3’- GATGGCGCGCCCTGAAAGTTAAAAATTACAGTATAAAGATA
AGGGA, and was subcloned into the EcoRV-AscI fragment from P5xQUAS-hsp70-GFP, replacing the hsp-70 minimal promoter.