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Punc-4::NGL-1::spGFP1-10
(Plasmid #65827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSM
  • Backbone manufacturer
    C.Bargmann
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 9503
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLG-1
  • Alt name
    NLG-1 cDNA
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2469
  • Entrez Gene
    nlg-1 (a.k.a. CELE_C40C9.5)
  • Promoter Punc-4
  • Tag / Fusion Protein
    • spGFP1-10 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer GCCAAAGGACCCAAAGGTATG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Miri K. VanHoven made it originally, named as MVC46

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Punc-4::NGL-1::spGFP1-10 was a gift from Cori Bargmann & Kang Shen (Addgene plasmid # 65827 ; http://n2t.net/addgene:65827 ; RRID:Addgene_65827)

  • For your References section:

    GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems. Feinberg EH, Vanhoven MK, Bendesky A, Wang G, Fetter RD, Shen K, Bargmann CI. Neuron. 2008 Feb 7. 57(3):353-63. 10.1016/j.neuron.2007.11.030 PubMed 18255029

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