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Addgene

pBT1-proD-mCherry-DAS+4
(Plasmid #65824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBT-1
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 3505
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-DAS+4
  • Species
    Synthetic
  • Insert Size (bp)
    1000
  • Promoter proD E . coli Constitutive Promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAG TCC AGT TAC GCT GGA GTC
  • 3′ sequencing primer GGT CAG GTA TGA TTT AA A TGG TCA GT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT1-proD-mCherry-DAS+4 was a gift from Michael Lynch (Addgene plasmid # 65824 ; http://n2t.net/addgene:65824 ; RRID:Addgene_65824)
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