pCASCADE
(Plasmid
#65821)
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PurposeControl guide RNA plasmid E. coli , low phosphate induction
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCASCADE
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Backbone manufacturerNone - Constructed in House
- Total vector size (bp) 2780
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecontrol guideRNA
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gRNA/shRNA sequenceNone- Control
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SpeciesSynthetic
- Promoter E . coli ugpB gene promoter, low phosphate induction
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GGGAGACCACAACGG
- 3′ sequencing primer AGTCCAGCTTGGAGCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCASCADE was a gift from Michael Lynch (Addgene plasmid # 65821 ; http://n2t.net/addgene:65821 ; RRID:Addgene_65821)
