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Addgene

pSMART-Ala1
(Plasmid #65814)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSMART-HCKan (AF532107.1)
  • Backbone manufacturer
    Lucigen (Middleton, WI)
  • Backbone size w/o insert (bp) 1800
  • Total vector size (bp) 3424
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NADPH Alanine Dehydrogenase
  • Alt name
    AlaDH
  • Species
    Synthetic
  • Insert Size (bp)
    1200
  • Mutation
    D196A, L197R
  • Entrez Gene
    ald (a.k.a. BSU31930)
  • Promoter Insulated waaHp from E. coli

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAG TCC AGT TAC GCT GGA GTC
  • 3′ sequencing primer GGT CAG GTA TGA TTT AA A TGG TCA GT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMART-Ala1 was a gift from Michael Lynch (Addgene plasmid # 65814 ; http://n2t.net/addgene:65814 ; RRID:Addgene_65814)

  • For your References section:

    Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion. Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD. Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440. 10.1002/bit.27440 PubMed 32441815
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