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pCI-MS2V5-eRF3a F76a
(Plasmid #65811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4572
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eRF3a
  • Species
    H. sapiens (human)
  • Mutation
    F76a
  • Entrez Gene
    GSPT1 (a.k.a. 551G9.2, ETF3A, GST1, eRF3a)
  • Promoter CMV
  • Tags / Fusion Proteins
    • MS2 stem loops (N terminal on insert)
    • V5 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-MS2V5-eRF3a F76a was a gift from Niels Gehring (Addgene plasmid # 65811 ; http://n2t.net/addgene:65811 ; RRID:Addgene_65811)

  • For your References section:

    The interaction of cytoplasmic poly(A)-binding protein with eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA decay. Fatscher T, Boehm V, Weiche B, Gehring NH. RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014 Aug 21. 10.1261/rna.044933.114 PubMed 25147240
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