pCI-MS2V5-PABPC1 MLLEmut M161A/D165K
(Plasmid #65810)

• Purpose: expression of PABPC1-MS2 fusion, double mutant.
• Depositing Lab: Niels Gehring
• Publication: Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014 Aug 21.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4572
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PABPC1
• Alt name: poly(A) binding protein, cytoplasmic 4
• Species: H. sapiens (human)
• Mutation: MLLEmut M161A/D165K
• Entrez Gene: PABPC4 (a.k.a. APP-1, APP1, PABP4, iPABP)
• Promoter: CMV
• Tags / Fusion Proteins:
  • MS2 stem loops (N terminal on insert)
  • V5 (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-R

Resource Information

• Supplemental Documents:
  • pCI-MS2V5-PABPC1 MLLEmut M161A/D165K genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry